Composite

Part:BBa_K3105678

Designed by: Jonas Gockel   Group: iGEM19_Uppsala_Universitet   (2019-10-11)


AAO-2A-eGFP

Part to co-express aryl-alcohol oxidase (AAO) and enhanced green-fluorescent protein (eGFP) in Pichia pastoris. The 2A-peptide will facilitate cleavage of the polypeptide during translation, leading to separate units of AAO and eGFP
To test the part, it was cloned into the pPICZαB vector and transformed into P. pastoris. The expression circuit and functional expression can be found under BBa_K3105680 .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 150
    Illegal BamHI site found at 337
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1297
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None